Unit

UNIT 8.3 Methods for Synchronizing Cells at Specific Stages of the Cell Cycle

  1. Joany Jackman1,
  2. Patrick M. O'Connor2

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb0803s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Jackman, J. and O'Connor, P. M. 2001. Methods for Synchronizing Cells at Specific Stages of the Cell Cycle. Current Protocols in Cell Biology. 00:8.3:8.3.1–8.3.20.

Author Information

  1. 1

    U.S.A.M.R.I.I.D., Fort Dietrick, Maryland

  2. 2

    Agouron Pharmaceuticals, San Diego, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998

Abstract

Exponentially growing cells are asynchronous with respect to the cell cycle stage. Detection of cell cycle-related events is improved by enriching the culture for cells at the stage during which the particular event occurs. Methods for synchronizing cells are provided here, including those based on morphological features of the cell (mitotic shake-off), cellular metabolism (thymidine inhibition, isoleucine depravation), and chemical inhibitors of cell progression in G1 (lovastatin), S (aphidicolin, mimosine), and G2/M (nocodazole). Applications of these methods and the advantages and disadvantages of each are described.