UNIT 9.2 Quantitative Measurement of Cell Adhesion Using Centrifugal Force
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
McClay, D. R. and Hertzler, P. L. 2001. Quantitative Measurement of Cell Adhesion Using Centrifugal Force. Current Protocols in Cell Biology. 00:9.2:9.2.1–9.2.10.
- Published Online: 1 MAY 2001
- Published Print: OCT 1998
In order to study the biophysical forces involved in cell-substrate (or cell-cell) adhesion, it is necessary to measure the strength of adhesion. Two questions can be addressed using the centrifugal cell adhesion assay provided in this unit: what is the ligand-receptor affinity for cells adhering at 4oC and what is the strength of the ATP-dependent processes that strengthen adhesion at 37oC. In both cases, the strength of adhesion is measured as the resistance to disruption in the presence of a measured centrifugal force.