Unit

UNIT 9.2 Quantitative Measurement of Cell Adhesion Using Centrifugal Force

  1. David R. McClay,
  2. Philip L. Hertzler

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb0902s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

McClay, D. R. and Hertzler, P. L. 2001. Quantitative Measurement of Cell Adhesion Using Centrifugal Force. Current Protocols in Cell Biology. 00:9.2:9.2.1–9.2.10.

Author Information

  1. Duke University, Durham, North Carolina

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998

Abstract

In order to study the biophysical forces involved in cell-substrate (or cell-cell) adhesion, it is necessary to measure the strength of adhesion. Two questions can be addressed using the centrifugal cell adhesion assay provided in this unit: what is the ligand-receptor affinity for cells adhering at 4oC and what is the strength of the ATP-dependent processes that strengthen adhesion at 37oC. In both cases, the strength of adhesion is measured as the resistance to disruption in the presence of a measured centrifugal force.