Unit

UNIT 9.4 Analyzing Integrin-Dependent Adhesion

  1. A. Paul Mould

Published Online: 1 DEC 2011

DOI: 10.1002/0471143030.cb0904s53

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Mould, A. P. 2011. Analyzing Integrin-Dependent Adhesion. Current Protocols in Cell Biology. 53:9.4:9.4.1–9.4.17.

Author Information

  1. University of Manchester, Manchester, United Kingdom

Publication History

  1. Published Online: 1 DEC 2011
  2. Published Print: DEC 2011

Abstract

In this unit, methods for the analysis of integrin-dependent adhesion are described. Two major types of assays are commonly used for this analysis. The first are cell adhesion assays (as described in unit 9.1). A key application of this type of assay is to identify which integrin(s) mediate cell-substrate interactions; a comprehensive list of antibodies suitable for this purpose is detailed. The second are solid-phase assays in which purified integrins and integrin ligands are used. These assays can be used, e.g., to measure apparent affinities of integrins for different ligands and IC50 values of pharmacological inhibitors. Curr. Protoc. Cell Biol. 53:9.4.1-9.4.17. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • cell biology;
  • adhesion;
  • extracellular matrix