UNIT 10.2 Preparation of Basement Membrane Components from EHS Tumors
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Kleinman, H. K. 2001. Preparation of Basement Membrane Components from EHS Tumors. Current Protocols in Cell Biology. 00:10.2:10.2.1–10.2.10.
- Published Online: 1 MAY 2001
- Published Print: OCT 1998
This unit describes methods for passaging and harvesting the basement membrane matrix-producing EHS tumor and for the subsequent isolation of a crude mixture of basement membrane components termed Matrigel, which promotes the differentiation of a variety of epithelial, endothelial, and neuronal cells. Procedures for the isolation of the adhesive glycoprotein laminin-1 and of type IV collagen are also included. Support protocols cover the maintenance and harvesting of EHS tumors in mice and maintenance of mice on a lathrogenic diet.