Unit

UNIT 10.2 Preparation of Basement Membrane Components from EHS Tumors

  1. Hynda K. Kleinman

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb1002s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Kleinman, H. K. 2001. Preparation of Basement Membrane Components from EHS Tumors. Current Protocols in Cell Biology. 00:10.2:10.2.1–10.2.10.

Author Information

  1. National Institute of Dental Research/NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998

Abstract

This unit describes methods for passaging and harvesting the basement membrane matrix-producing EHS tumor and for the subsequent isolation of a crude mixture of basement membrane components termed Matrigel, which promotes the differentiation of a variety of epithelial, endothelial, and neuronal cells. Procedures for the isolation of the adhesive glycoprotein laminin-1 and of type IV collagen are also included. Support protocols cover the maintenance and harvesting of EHS tumors in mice and maintenance of mice on a lathrogenic diet.