Unit

UNIT 10.4 Preparation of Extracellular Matrices Produced by Cultured Corneal Endothelial and PF-HR9 Endodermal Cells

  1. Israel Vlodavsky

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb1004s01

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Vlodavsky, I. 2001. Preparation of Extracellular Matrices Produced by Cultured Corneal Endothelial and PF-HR9 Endodermal Cells. Current Protocols in Cell Biology. 1:10.4:10.4.1–10.4.14.

Author Information

  1. Hadassah-Hebrew University Hospital, Jerusalem, Israel

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 1999

Abstract

The ECM is an organized complex of collagens, proteoglycans, and glycoproteins, all interacting to produce a highly stable structure upon which cells migrate, proliferate, differentiate, and survive in vivo. Cultured bovine corneal endothelial (BCE) cells and PF-HR9 endodermal cells produce underlying ECMs that adhere strongly to plastic and closely resemble subendothelial and subepithelial basement membranes in vivo in their morphology, molecular composition, and biological activities. This unit describes the methods for preparation of these ECMs, their properties, and their cellular effects.