Unit

UNIT 10.5 Purification of Fibronectin

  1. Steven K. Akiyama

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb1005s02

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Akiyama, S. K. 2001. Purification of Fibronectin. Current Protocols in Cell Biology. 2:10.5:10.5.1–10.5.13.

Author Information

  1. Chapel Hill, North Carolina

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1999

This is not the most recent version of the article. View current version (24 SEP 2013)

Abstract

This unit describes the purification of the multifunctional adhesive glycoprotein fibronectin from plasma or of cell-derived fibronectin from cell surfaces and from conditioned medium. Fibronectin can be used in cell adhesion and migration assays, and can be obtained in relatively high purity using simple affinity chromatography techniques.