Miscellaneous
UNIT 10.7 Proteoglycan Isolation and Analysis
Published Online: 1 MAY 2001
DOI: 10.1002/0471143030.cb1007s07
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
Additional Information
How to Cite
Woods, A. and Couchman, J. R. 2001. Proteoglycan Isolation and Analysis. Current Protocols in Cell Biology. 10.7.1–10.7.19.
Publication History
- Published Online: 1 MAY 2001
- Published Print: JUL 2000
- Abstract
- Article
- References
Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of glycoaminoglycan size and type and of core protein species are described.