UNIT 10.8 Matrix Metalloproteinases
Published Online: 1 SEP 2008
Copyright © 2008 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Birkedal-Hansen, H., Yamada, S., Windsor, J., Pollard, A. H., Lyons, G., Stetler-Stevenson, W. and Birkedal-Hansen, B. 2008. Matrix Metalloproteinases. Current Protocols in Cell Biology. 40:10.8:10.8.1–10.8.23.
- Published Online: 1 SEP 2008
- Published Print: SEP 2008
Matrix metalloproteinases are a class of enzymes that play an important role in the remodeling of the extracellular matrix in development and cancer metastasis. This unit describes a set of methods—cell-mediated dissolution of type-1 collagen fibrils, direct and reverse zymography, enzyme capture based on α2-macroglobulin and TIMP-1 and -2, and demonstration of cryptic thiol groups in metalloproteinase precursors—that are used to characterize the functions of matrix metalloproteinases and their inhibitors. Curr. Protoc. Cell Biol. 40:10.8.1-10.8.23. © 2008 by John Wiley & Sons, Inc.
- matrix metalloproteinases;
- type-1 collagen;
- TIMP-1 and -2