Unit

UNIT 10.11 Purification of SPARC/Osteonectin

  1. E. Helene Sage

Published Online: 1 FEB 2003

DOI: 10.1002/0471143030.cb1011s17

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Sage, E. H. 2003. Purification of SPARC/Osteonectin. Current Protocols in Cell Biology. 17:10.11:10.11.1–10.11.23.

Author Information

  1. The Hope Heart Institute, Seattle, Washington

Publication History

  1. Published Online: 1 FEB 2003
  2. Published Print: JAN 2003

Abstract

SPARC is a matricellular protein that regulates cell adhesion, extracelluolar matrix production, growth factor activity and cell cycle. This unit describes the purification of SPARC, also termed osteonectin and BM/40, from cultured mammalian cells. Additional information is presented on the purification of recombinant SPARC (rSPARC) from E. coli and from Sf9 cells, as well as its isolation from blood platelets. Assays for the activity of SPARC, de-adhesion and inhibition of cellular proliferation in vitro, are described. The expression of SPARC during remodeling and repair tissue in response to injury identifies it as a therapeutic target for the treatment of fibrotic disease, certain cancers and other disorders in which regulation of angiogenesis is a key factor.