Unit

UNIT 10.13 Non-Radioactive Quantification of Fibronectin Matrix Assembly

  1. Roumen Pankov,
  2. Kenneth M. Yamada

Published Online: 1 DEC 2004

DOI: 10.1002/0471143030.cb1013s25

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Pankov, R. and Yamada, K. M. 2004. Non-Radioactive Quantification of Fibronectin Matrix Assembly. Current Protocols in Cell Biology. 25:10.13:10.13.1–10.13.9.

Author Information

  1. National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 DEC 2004
  2. Published Print: NOV 2004

Abstract

This unit provides a basic protocol for non-radioactive determination of the rate of incorporation of biotinylated fibronectin into the insoluble matrix organized by cultured cells. The protocol provides quantification of changes in matrix assembly due to different experimental conditions with concomitant determinations of the activation state of various signaling molecules that may be involved in the process of matrix assembly. This unit also provides a support protocol for biotinylation of purified fibronectin.

Keywords:

  • fibronectin;
  • biotinylation;
  • deoxycholate;
  • solubility;
  • phosphospecific antibodies