UNIT 10.17 Engineering Three-Dimensional Collagen Matrices to Provide Contact Guidance during 3D Cell Migration
Published Online: 1 JUN 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Provenzano, P. P., Eliceiri, K. W., Inman, D. R. and Keely, P. J. 2010. Engineering Three-Dimensional Collagen Matrices to Provide Contact Guidance during 3D Cell Migration. Current Protocols in Cell Biology. 47:10.17:10.17.1–10.17.11.
- Published Online: 1 JUN 2010
- Published Print: JUN 2010
Cell invasion requires that cells navigate complex three-dimensional matrices in vivo. Topological cues are provided and three-dimensional cell migration and invasion facilitated by the alignment of collagen fibers proximal to tumors. In order to better understand the molecular mechanisms by which cells recognize and migrate along aligned matrices, in vitro assays are needed that recapitulate topological features of the in vivo matrix. Here, we describe two approaches for creating aligned three-dimensional collagen matrices, both dependent and independent of cell-mediated alignment. Approaches to quantify alignment and visualize the collagen matrix relative to the cells by second-harmonic generation are included. These assays are readily adaptable to a variety of cells and biological questions related to three-dimensional cell migration. Curr. Protoc. Cell Biol. 47:10.17.1-10.17.11. © 2010 by John Wiley & Sons, Inc.
- collagen alignment;
- three-dimensional cell migration;