Unit

UNIT 11.1 Overview of Eukaryotic In Vitro Translation and Expression Systems

  1. Rosemary Jagus1,
  2. Gregory S. Beckler2

Published Online: 1 APR 2003

DOI: 10.1002/0471143030.cb1101s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Jagus, R. and Beckler, G. S. 2003. Overview of Eukaryotic In Vitro Translation and Expression Systems. Current Protocols in Cell Biology. 00:11.1:11.1.1–11.1.13.

Author Information

  1. 1

    Center of Marine and Biotechnology and Greenebaum Cancer Center, Baltimore, Maryland

  2. 2

    Promega Corporation, Madison, Wisconsin

Publication History

  1. Published Online: 1 APR 2003
  2. Published Print: MAR 2003

Abstract

The ability to investigate cellular processes in vitro permits detailed analysis of the process and its molecular components. Eukaryotic translation and expression is one system that has been well studied. This overview describes the development of in vitro systems, including such approaches as continuous-flow systems, coupled transcription/translation, and the incorporation of non-natural amino acids. It also discusses molecular and genetic studies to probe translation, including post-translational fate of the synthesized proteins.