UNIT 11.2 In Vitro Translation
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Jagus, R., Joshi, B., Miyamoto, S. and Beckler, G. S. 2001. In Vitro Translation. Current Protocols in Cell Biology. 00:11.2:11.2.1–11.2.25.
- Published Online: 1 MAY 2001
- Published Print: OCT 1998
The two most frequently used systems for in vitro translation are the rabbit reticulocyte system and the wheat germ extract. These systems are useful for mRNAs isolated from cells, tissues, and capped or uncapped mRNA produced in vitro by transcription of cDNA. In a combined system, mRNA can be transcribed and translated in a single reaction. In addition these systems can be used for translation reactions with biotinylated amino acids; this allows capture of the newly synthesized protein using streptavidin immobilized on agarose.