UNIT 11.2 In Vitro Translation

  1. Rosemary Jagus1,
  2. Bhavesh Joshi2,
  3. Suzzane Miyamoto3,
  4. Gregory S. Beckler4

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb1102s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Jagus, R., Joshi, B., Miyamoto, S. and Beckler, G. S. 2001. In Vitro Translation. Current Protocols in Cell Biology. 00:11.2:11.2.1–11.2.25.

Author Information

  1. 1

    Center of Marine Biotechnology and Greenebaum Cancer Center, Baltimore, Maryland

  2. 2

    Center of Marine Biotechnology, Baltimore, Maryland

  3. 3

    Molecular Hematology Branch, NHLBI, Bethesda, Maryland

  4. 4

    Promega Corporation, Madison, Wisconsin

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998


The two most frequently used systems for in vitro translation are the rabbit reticulocyte system and the wheat germ extract. These systems are useful for mRNAs isolated from cells, tissues, and capped or uncapped mRNA produced in vitro by transcription of cDNA. In a combined system, mRNA can be transcribed and translated in a single reaction. In addition these systems can be used for translation reactions with biotinylated amino acids; this allows capture of the newly synthesized protein using streptavidin immobilized on agarose.