Unit

UNIT 11.3 In Vitro Analysis of Endoplasmic-Reticulum-to-Golgi Transport in Mammalian Cells

  1. Bernard B. Allan,
  2. William E. Balch

Published Online: 1 APR 2001

DOI: 10.1002/0471143030.cb1103s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Allan, B. B. and Balch, W. E. 2001. In Vitro Analysis of Endoplasmic-Reticulum-to-Golgi Transport in Mammalian Cells. Current Protocols in Cell Biology. 00:11.3:11.3.1–11.3.22.

Author Information

  1. The Scripps Research Institute, La Jolla, California

Publication History

  1. Published Online: 1 APR 2001
  2. Published Print: OCT 1998

Abstract

A temperature-sensitive mutant of vesicular stomatitis G protein is used to follow the movement of that protein from the endoplasmic reticulum to transport vesicles to cis-Golgi and finally medial/trans-Golgi by assessing the maturation of two asparagine-linked oligosaccharides. These assays can be used to identify the factors that are required for and regulate protein trafficking through these compartments.