Unit

UNIT 11.4 Cotranslational Translocation of Proteins into Canine Rough Microsomes

  1. Harris D. Bernstein

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb1104s00

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Bernstein, H. D. 2001. Cotranslational Translocation of Proteins into Canine Rough Microsomes. Current Protocols in Cell Biology. 00:11.4:11.4.1–11.4.11.

Author Information

  1. National Institute of Diabetes and Digestive and Kidney Disesases/NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998

Abstract

Rough microsomes (RMs) are small vesicular fragments of the rough endoplasmic reticulum (ER). They can be used in cell-free protein translocation studies to analyze ER targeting signals, signal sequence cleavage, N-linked glycosylation, and membrane protein biogenesis. This unit describes preparation and use of RMs.Rough microsomes (RMs) are small vesicular fragments of the rough endoplasmic reticulum (ER). They can be used in cell-free protein translocation studies to analyze ER targeting signals, signal sequence cleavage, N-linked glycosylation, and membrane protein biogenesis. This unit describes preparation and use of RMs.