UNIT 11.5 In Vitro Analysis of SV40 DNA Replication
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Yin, Y.-L. and Dutta, A. 2001. In Vitro Analysis of SV40 DNA Replication. Current Protocols in Cell Biology. 2:11.5:11.5.1–11.5.12.
- Published Online: 1 MAY 2001
- Published Print: APR 1999
This unit describes a method for studying cellular factors that affect eukaryotic replication by using a plasmid that contains an SV40 origin of replication and supplying the viral helicase T antigen. Replication factors are supplied by S100 extracts of 293 cells. The unit also contains protocols for expression and purification of T antigen and analysis of the products of replication.