Unit

UNIT 11.5 In Vitro Analysis of SV40 DNA Replication

  1. Yi-Ling Yin1,
  2. Anindya Dutta2

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb1105s02

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Yin, Y.-L. and Dutta, A. 2001. In Vitro Analysis of SV40 DNA Replication. Current Protocols in Cell Biology. 2:11.5:11.5.1–11.5.12.

Author Information

  1. 1

    Genetics Institute, Cambridge, Massachusetts

  2. 2

    Brigham and Women's Hospital, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1999

Abstract

This unit describes a method for studying cellular factors that affect eukaryotic replication by using a plasmid that contains an SV40 origin of replication and supplying the viral helicase T antigen. Replication factors are supplied by S100 extracts of 293 cells. The unit also contains protocols for expression and purification of T antigen and analysis of the products of replication.