Unit

UNIT 11.9 Analysis of Eukaryotic Translation in Purified and Semipurified Systems

  1. William C. Merrick,
  2. Jack O. Hensold

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb1109s08

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Merrick, W. C. and Hensold, J. O. 2001. Analysis of Eukaryotic Translation in Purified and Semipurified Systems. Current Protocols in Cell Biology. 8:11.9:11.9.1–11.9.26.

Author Information

  1. Case Western Reserve University School of Medicine, Cleveland, Ohio

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 2000

Abstract

Much of the current understanding of the sequential steps involved in translation initiation has been obtained using sucrose gradients to isolate ribosomes and ribosomal subunits, as described here. These purified components are combined with purified translation factors to analyze the formation of intermediates in translation initiation and the roles of the translation factors in vitro.