UNIT 11.9 Analysis of Eukaryotic Translation in Purified and Semipurified Systems
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Merrick, W. C. and Hensold, J. O. 2001. Analysis of Eukaryotic Translation in Purified and Semipurified Systems. Current Protocols in Cell Biology. 8:11.9:11.9.1–11.9.26.
- Published Online: 1 MAY 2001
- Published Print: OCT 2000
Much of the current understanding of the sequential steps involved in translation initiation has been obtained using sucrose gradients to isolate ribosomes and ribosomal subunits, as described here. These purified components are combined with purified translation factors to analyze the formation of intermediates in translation initiation and the roles of the translation factors in vitro.