Unit

UNIT 11.13 Mitotic Spindle Assembly In Vitro

  1. John Merlie Jr.,
  2. Rebecca Heald

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb1113s09

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Merlie, J. and Heald, R. 2001. Mitotic Spindle Assembly In Vitro. Current Protocols in Cell Biology. 9:11.13:11.13.1–11.13.23.

Author Information

  1. University of California, Berkeley, Berkeley, Carolina

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 2001

Abstract

The protocols in this unit describe the preparation of materials for an in vitro assay of mitotic spindle assembly in Xenopus egg extracts. Fluorochrome-labeled tubulin is used to visualize microtubule asters and spindles.