UNIT 11.14 Analysis of RNA Export Using Xenopus Oocytes
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Duricka, D. and Ullman, K. S. 2001. Analysis of RNA Export Using Xenopus Oocytes. Current Protocols in Cell Biology. 10:11.14:11.14.1–11.14.13.
- Published Online: 1 MAY 2001
- Published Print: APR 2001
This unit describes a procedure for monitoring RNA export in Xenopus oocytes. The technique involves synthesizing labeled RNA in vitro and microinjecting the RNA into oocyte nuclei. Following incubation the oocytes are dissected into nuclear and cytoplasmic fractions. These samples are then processed for RNA analysis, allowing the extent of export to be quantitatively assessed.