Unit

UNIT 11.14 Analysis of RNA Export Using Xenopus Oocytes

  1. Deborah Duricka,
  2. Katharine S. Ullman

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb1114s10

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Duricka, D. and Ullman, K. S. 2001. Analysis of RNA Export Using Xenopus Oocytes. Current Protocols in Cell Biology. 10:11.14:11.14.1–11.14.13.

Author Information

  1. University of Utah, Salt Lake City, Utah

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2001

Abstract

This unit describes a procedure for monitoring RNA export in Xenopus oocytes. The technique involves synthesizing labeled RNA in vitro and microinjecting the RNA into oocyte nuclei. Following incubation the oocytes are dissected into nuclear and cytoplasmic fractions. These samples are then processed for RNA analysis, allowing the extent of export to be quantitatively assessed.