UNIT 11.15 In Vitro Analysis of Peroxisomal Protein Import
Published Online: 1 MAY 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Terlecky, S. R. 2002. In Vitro Analysis of Peroxisomal Protein Import. Current Protocols in Cell Biology. 14:11.15:11.15.1–11.15.10.
- Published Online: 1 MAY 2002
- Published Print: APR 2002
This unit describes a quantitative in vitro assay for peroxisomal protein import that accurately reconstitutes established properties of the pathway. The cell-free system is ELISA based and employs semi-permeabilized human cells with a biotinylated peroxisomal targeting signal-containing substrate.