Unit

UNIT 11.15 In Vitro Analysis of Peroxisomal Protein Import

  1. Stanley R. Terlecky

Published Online: 1 MAY 2002

DOI: 10.1002/0471143030.cb1115s14

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Terlecky, S. R. 2002. In Vitro Analysis of Peroxisomal Protein Import. Current Protocols in Cell Biology. 14:11.15:11.15.1–11.15.10.

Author Information

  1. Wayne State University School of Medicine, Detroit, Michigan

Publication History

  1. Published Online: 1 MAY 2002
  2. Published Print: APR 2002

Abstract

This unit describes a quantitative in vitro assay for peroxisomal protein import that accurately reconstitutes established properties of the pathway. The cell-free system is ELISA based and employs semi-permeabilized human cells with a biotinylated peroxisomal targeting signal-containing substrate.