Unit

UNIT 12.6 Optical Microscopy–Based Migration Assay for Human Neutrophils

  1. Lynda M. Pierini,
  2. Frederick R. Maxfield

Published Online: 1 FEB 2003

DOI: 10.1002/0471143030.cb1206s17

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Pierini, L. M. and Maxfield, F. R. 2003. Optical Microscopy–Based Migration Assay for Human Neutrophils. Current Protocols in Cell Biology. 17:12.6:12.6.1–12.6.15.

Author Information

  1. Weill Medical College of Cornell University, New York, New York

Publication History

  1. Published Online: 1 FEB 2003
  2. Published Print: JAN 2003

Abstract

This unit describes an in vitro microscopy assay for examining the migration of human neutrophils in two dimensions to identify the underlying cause of a migration defect and to evaluate a variety of migration parameters that cannot be studied using migration through a porous filter. Freshly isolated human neutrophils a placed in the chamber, stimulated, and images are collected at various time points. The data can be used to determine the effects of a pharmacological treatment on the migration of individual cells.