UNIT 13.2 In Vitro Motility Assay to Study Translocation of Actin by Myosin
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Sellers, J. R. 2001. In Vitro Motility Assay to Study Translocation of Actin by Myosin. Current Protocols in Cell Biology. 00:13.2:13.2.1–13.2.10.
- Published Online: 1 MAY 2001
- Published Print: OCT 1998
A basic property of myosin is its ability to interact with and translocate actin. This unit describes an in vitro motility assay that can be used to study the translocation, or sliding, of actin filaments by myosin bound to a coverslip. The assay makes use of the ability to image single F-actin filaments labeled with rhodamine phalloidin, a high-affinity fluorescent ligand using fluorescence microscopy. The system is fast, easy to set up and maintain, uses only small amounts of protein, and yields quantitative results.