UNIT 13.3 Organelle Motility in Plant Cells: Imaging Golgi and ER Dynamics with GFP
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Hawes, C., Brandizzil, F., Batoko, H. and Moore, I. 2001. Organelle Motility in Plant Cells: Imaging Golgi and ER Dynamics with GFP. Current Protocols in Cell Biology. 9:13.3:13.3.1–13.3.10.
- Published Online: 1 MAY 2001
- Published Print: JAN 2001
By the addition of plant or mammalian targeting sequences, green fluorescent protein (GFP) can be directed to the endoplasmic reticulum, the Golgi apparatus, or both organelles in plant cells. This unit describes the application of rapid Agrobacterium and virus-mediated transient expression systems in leaf tissue to permit visualization of ER and Golgi dynamics in vivo by epifluorescence or confocal microscopy.