Unit

UNIT 13.3 Organelle Motility in Plant Cells: Imaging Golgi and ER Dynamics with GFP

  1. Chris Hawes1,
  2. Federica Brandizzil1,
  3. Henri Batoko2,
  4. Ian Moore2

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb1303s09

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Hawes, C., Brandizzil, F., Batoko, H. and Moore, I. 2001. Organelle Motility in Plant Cells: Imaging Golgi and ER Dynamics with GFP. Current Protocols in Cell Biology. 9:13.3:13.3.1–13.3.10.

Author Information

  1. 1

    Oxford Brookes University, Oxford, United Kingdom

  2. 2

    University of Oxford, Oxford, United Kingdom

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 2001

Abstract

By the addition of plant or mammalian targeting sequences, green fluorescent protein (GFP) can be directed to the endoplasmic reticulum, the Golgi apparatus, or both organelles in plant cells. This unit describes the application of rapid Agrobacterium and virus-mediated transient expression systems in leaf tissue to permit visualization of ER and Golgi dynamics in vivo by epifluorescence or confocal microscopy.