Unit

UNIT 13.5 Measuring Dynamics of Nuclear Proteins by Photobleaching

  1. Miroslav Dundr,
  2. Tom Misteli

Published Online: 1 MAY 2003

DOI: 10.1002/0471143030.cb1305s18

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Dundr, M. and Misteli, T. 2003. Measuring Dynamics of Nuclear Proteins by Photobleaching. Current Protocols in Cell Biology. 18:13.5:13.5.1–13.5.18.

Author Information

  1. National Cancer Institute, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2003
  2. Published Print: MAR 2003

Abstract

The mobility of nuclear proteins can be studied by photobleaching techniques. The three main advantages of photobleaching are fast experimental turn around, good spatial and temporal resolution, and the ability to measure kinetics inside of living cells. The main disadvantage of these techniques is the requirement for fluorescently tagged proteins that have rigorously tested to ensure it has the same properties and function as its native counterpart. Three major methods of photobleaching microscopy are described: fluorescence recovery after photobleaching (FRAP), fluorescence loss in photobleaching (FLIP), and inverse fluorescence recovery after photobleaching (iFRAP). Each of these techniques has characteristics permitting the determination of distinct parameters of protein behavior in vivo.