Unit

UNIT 14.4 Labeling Cultured Cells with 32Pi and Preparing Cell Lysates for Immunoprecipitation

  1. Bartholomew M. Sefton

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb1404s03

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Sefton, B. M. 2001. Labeling Cultured Cells with 32Pi and Preparing Cell Lysates for Immunoprecipitation. Current Protocols in Cell Biology. 3:14.4:14.4.1–14.4.7.

Author Information

  1. The Salk Institute, San Diego, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1999

Abstract

Signal transduction pathways may involve protein phosphorylation at one or more residues. Detection of phosphorylation involves labeling with radioactive inorganic phosphate and subsequent immunoprecipitation with appropriate antibodies. This unit describes labeling conditions for adherent and nonadherent cells and preparation of the lysates from these cells for immunoprecipitation. The labeling method is appropriate for labeling other phosphorylated component of cells, but alternate methods are necessary for their detection.