UNIT 14.4 Labeling Cultured Cells with 32Pi and Preparing Cell Lysates for Immunoprecipitation
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Sefton, B. M. 2001. Labeling Cultured Cells with 32Pi and Preparing Cell Lysates for Immunoprecipitation. Current Protocols in Cell Biology. 3:14.4:14.4.1–14.4.7.
- Published Online: 1 MAY 2001
- Published Print: JUL 1999
Signal transduction pathways may involve protein phosphorylation at one or more residues. Detection of phosphorylation involves labeling with radioactive inorganic phosphate and subsequent immunoprecipitation with appropriate antibodies. This unit describes labeling conditions for adherent and nonadherent cells and preparation of the lysates from these cells for immunoprecipitation. The labeling method is appropriate for labeling other phosphorylated component of cells, but alternate methods are necessary for their detection.