UNIT 14.5 Phosphoamino Acid Analysis

  1. Bartholomew.M. Sefton

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb1405s03

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Sefton, B. 2001. Phosphoamino Acid Analysis. Current Protocols in Cell Biology. 3:14.5:14.5.1–14.5.8.

Author Information

  1. The Salk Institute, San Diego, Carolina

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1999


Proteins involved in signal transduction are often phosphorylated. Determination of the specific amino acid residue(s) involved is used in characterizing the particular pathway. Partial acid hydrolysis of phosphorylated proteins followed by two-dimensional thin layer chromatography is used to identify the phosphorylated residues of the protein as phosphoserine, phosphothreonine, or phosphotyrosine. Mild alkaline hydrolysis is used to enhance detection of phosphothreonine and phosphotyrosine.