UNIT 14.5 Phosphoamino Acid Analysis
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Sefton, B. 2001. Phosphoamino Acid Analysis. Current Protocols in Cell Biology. 3:14.5:14.5.1–14.5.8.
- Published Online: 1 MAY 2001
- Published Print: JUL 1999
Proteins involved in signal transduction are often phosphorylated. Determination of the specific amino acid residue(s) involved is used in characterizing the particular pathway. Partial acid hydrolysis of phosphorylated proteins followed by two-dimensional thin layer chromatography is used to identify the phosphorylated residues of the protein as phosphoserine, phosphothreonine, or phosphotyrosine. Mild alkaline hydrolysis is used to enhance detection of phosphothreonine and phosphotyrosine.