UNIT 14.6 Determination of Akt/PKB Signaling
Published Online: 1 MAY 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Pankov, R. 2004. Determination of Akt/PKB Signaling. Current Protocols in Cell Biology. 22:14.6:14.6.1–14.6.12.
- Published Online: 1 MAY 2004
- Published Print: MAR 2004
This unit provides a basic protocol for assaying the level of activity, as well as the activation capacity and dynamics of inhibition of Akt/PKB in cultured cells. All these data can be obtained in a single nonradioactive experiment by standard techniques including immunoblotting and immunodetection with phosphospecific antibodies.
This unit also provides a support protocol for assaying the membrane translocation of Akt.
- protein kinase B (PKB);
- okadaic acid;
- phosphospecific antibodies