Unit

UNIT 14.6 Determination of Akt/PKB Signaling

  1. Roumen Pankov

Published Online: 1 MAY 2004

DOI: 10.1002/0471143030.cb1406s22

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Pankov, R. 2004. Determination of Akt/PKB Signaling. Current Protocols in Cell Biology. 22:14.6:14.6.1–14.6.12.

Author Information

  1. National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2004
  2. Published Print: MAR 2004

Abstract

This unit provides a basic protocol for assaying the level of activity, as well as the activation capacity and dynamics of inhibition of Akt/PKB in cultured cells. All these data can be obtained in a single nonradioactive experiment by standard techniques including immunoblotting and immunodetection with phosphospecific antibodies.

This unit also provides a support protocol for assaying the membrane translocation of Akt.

Keywords:

  • Akt;
  • protein kinase B (PKB);
  • okadaic acid;
  • phosphospecific antibodies