Unit

UNIT 14.11 Biosensors for Characterizing the Dynamics of Rho Family GTPases in Living Cells

  1. Louis Hodgson1,
  2. Feimo Shen2,
  3. Klaus Hahn2

Published Online: 1 MAR 2010

DOI: 10.1002/0471143030.cb1411s46

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Hodgson, L., Shen, F. and Hahn, K. 2010. Biosensors for Characterizing the Dynamics of Rho Family GTPases in Living Cells. Current Protocols in Cell Biology. 46:14.11:14.11.1–14.11.26.

Author Information

  1. 1

    Gruss-Lipper Biophotonics Center, Department of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York

  2. 2

    University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

Publication History

  1. Published Online: 1 MAR 2010
  2. Published Print: MAR 2010

Abstract

The biosensors developed in the authors' laboratory have been based on different designs, each imparting specific strengths and weaknesses. Here we describe detailed protocols for the application of three biosensors exemplifying different designs—first, a design in which an environmentally sensitive dye is used to report the activation of endogenous Cdc42, followed by two biosensors based on FRET, one using intramolecular and the other intermolecular FRET. The design differences lead to the need for different approaches in imaging and image analysis. Curr. Protoc. Cell Biol. 46:14.11.1-14.11.26. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • biosensors;
  • Rho family GTPases;
  • FRET;
  • live cell imaging