Unit

UNIT 15.2 Use of Glycosidases to Study Protein Trafficking

  1. Hudson H. Freeze

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb1502s03

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Freeze, H. H. 2001. Use of Glycosidases to Study Protein Trafficking. Current Protocols in Cell Biology. 3:15.2:15.2.1–15.2.26.

Author Information

  1. The Burnham Institute, La Jolla, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1999

Abstract

As proteins transit through the cell secretory pathway, modification of their substituent sugar chains occurs in a stepwise fashion. In the course of this processing (maturation) of oligosaccharide chains, the chains acquire sensitivity or resistance to highly specific glycosidases. Thus it is possible to identify processing mileposts by analyzing the general structure of the carbohydrate chains. This unit describes reaction conditions for the family of glycosidases and analysis of the results of digestion reactions.