UNIT 15.2 Use of Glycosidases to Study Protein Trafficking
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Freeze, H. H. 2001. Use of Glycosidases to Study Protein Trafficking. Current Protocols in Cell Biology. 3:15.2:15.2.1–15.2.26.
- Published Online: 1 MAY 2001
- Published Print: JUL 1999
As proteins transit through the cell secretory pathway, modification of their substituent sugar chains occurs in a stepwise fashion. In the course of this processing (maturation) of oligosaccharide chains, the chains acquire sensitivity or resistance to highly specific glycosidases. Thus it is possible to identify processing mileposts by analyzing the general structure of the carbohydrate chains. This unit describes reaction conditions for the family of glycosidases and analysis of the results of digestion reactions.