UNIT 15.15 Documenting GLUT4 Exocytosis and Endocytosis in Muscle Cell Monolayers
Published Online: 1 MAR 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Ishikura, S., Antonescu, C. N. and Klip, A. 2010. Documenting GLUT4 Exocytosis and Endocytosis in Muscle Cell Monolayers. Current Protocols in Cell Biology. 46:15.15:15.15.1–15.15.19.
- Published Online: 1 MAR 2010
- Published Print: MAR 2010
The elevated blood glucose following a meal is cleared by insulin-stimulated glucose entry into muscle and fat cells. The hormone increases the amount of the glucose transporter GLUT4 at the plasma membrane in these tissues at the expense of preformed intracellular pools. In addition, muscle contraction also increases glucose uptake via a gain in GLUT4 at the plasma membrane. Regulation of GLUT4 levels at the cell surface could arise from alterations in the rate of its exocytosis, endocytosis, or both. Hence, methods that can independently measure these traffic parameters for GLUT4 are essential to understanding the mechanism of regulation of membrane traffic of the transporter. Here, we describe cell population–based assays to measure the steady-state levels of GLUT4 at the cell surface, as well as to separately measure the rates of GLUT4 endocytosis and endocytosis. Curr. Protoc. Cell Biol. 46:15.15.1-15.15.19. © 2010 by John Wiley & Sons, Inc.
- muscle contraction;
- regulated membrane traffic