UNIT 15.19 Synchronizing Protein Transport in the Secretory Pathway
Published Online: 1 DEC 2012
Copyright © 2012 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Boncompain, G. and Perez, F. 2012. Synchronizing Protein Transport in the Secretory Pathway. Current Protocols in Cell Biology. 57:15.19:15.19.1–15.19.16.
- Published Online: 1 DEC 2012
- Published Print: DEC 2012
To be secreted or transported to their target compartments, newly synthesized proteins leave the endoplasmic reticulum to reach the Golgi apparatus, where they are processed and sorted toward their final destinations along the secretory pathway. It is now clear that many Golgi-intersecting and non-intersecting pathways exist in cells to carry out proper transport, modification, and addressing. To analyze and visualize the intracellular trafficking of any secretory protein, we developed the retention using selective hooks (RUSH) system. This assay allows the simultaneous release of a pool of particular secretory proteins from the endoplasmic reticulum and the monitoring of their anterograde trafficking. The use of the RUSH system is detailed in these protocols, from sub-cloning the sequence coding for the protein of interest into RUSH plasmids to visualization of its trafficking. Curr. Protoc. Cell Biol. 57:15.19.1-15.19.16. © 2012 by John Wiley & Sons, Inc.
- intracellular trafficking;
- secretory pathway