UNIT 15.19 Synchronizing Protein Transport in the Secretory Pathway

  1. Gaelle Boncompain1,2,
  2. Franck Perez1,2

Published Online: 1 DEC 2012

DOI: 10.1002/0471143030.cb1519s57

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Boncompain, G. and Perez, F. 2012. Synchronizing Protein Transport in the Secretory Pathway. Current Protocols in Cell Biology. 57:15.19:15.19.1–15.19.16.

Author Information

  1. 1

    Research Center, Institut Curie, Paris, France

  2. 2

    CNRS UMR144, Paris, France

Publication History

  1. Published Online: 1 DEC 2012
  2. Published Print: DEC 2012


To be secreted or transported to their target compartments, newly synthesized proteins leave the endoplasmic reticulum to reach the Golgi apparatus, where they are processed and sorted toward their final destinations along the secretory pathway. It is now clear that many Golgi-intersecting and non-intersecting pathways exist in cells to carry out proper transport, modification, and addressing. To analyze and visualize the intracellular trafficking of any secretory protein, we developed the retention using selective hooks (RUSH) system. This assay allows the simultaneous release of a pool of particular secretory proteins from the endoplasmic reticulum and the monitoring of their anterograde trafficking. The use of the RUSH system is detailed in these protocols, from sub-cloning the sequence coding for the protein of interest into RUSH plasmids to visualization of its trafficking. Curr. Protoc. Cell Biol. 57:15.19.1-15.19.16. © 2012 by John Wiley & Sons, Inc.


  • intracellular trafficking;
  • secretory pathway