UNIT 16.2 Production of Polyclonal Antisera
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Cooper, H. M. and Paterson, Y. 2001. Production of Polyclonal Antisera. Current Protocols in Cell Biology. 3:16.2:16.2.1–16.2.8.
- Published Online: 1 MAY 2001
- Published Print: JUL 1999
Polyclonal antibody preparations contain multiple antibody molecules recognizing different epitopes on an antigen molecule. Good quality polyclonal antisera are useful for a number of applications–e.g., immunoprecipitation, immunoblotting, and ELISAs–where the binding of more than one antibody molecule per antigen molecule improves the sensitivity of the assay. It is possible to produce polyclonal antibodies in a variety of species, and this make it possible to detect multiple immunoreactions. The protocols in this unit describe the production of polyclonal antisera specific for protein antigens in rabbits, rats, mice, and hamsters using complete Freund's adjuvant (CFA) or other adjuvants. In addition, the unit presents a method for preparing serum from blood. Polyclonal antipeptide antisera can be produced by substituting carrier-conjugated peptides for the purified protein antigens.