UNIT 16.3 Purification of Immunoglobulin G
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Andrew, S. M. and Titus, J. A. 2001. Purification of Immunoglobulin G. Current Protocols in Cell Biology. 5:16.3:16.3.1–16.3.12.
- Published Online: 1 MAY 2001
- Published Print: JAN 2000
While unpurified antibodies are suitable for a number of applications, purified antibodies are required for assays based on known concentration of antibody, for chemical modifications such as radiolabeling or conjugation with fluorochromes, or for structural modifications such as production of F(ab')2 or monvalent Fab fragments. This unit contains protocols for purification of IgG by ammonium sulfate precipitation coupled with size-exclusion chromatography, Protein A- and Protein G-affinity chromatography, immunoaffinity chromatography, and ion-exchange chromatography.