Unit

UNIT 16.3 Purification of Immunoglobulin G

  1. Sarah M. Andrew1,
  2. Julie A. Titus2

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb1603s05

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Andrew, S. M. and Titus, J. A. 2001. Purification of Immunoglobulin G. Current Protocols in Cell Biology. 5:16.3:16.3.1–16.3.12.

Author Information

  1. 1

    Chester College, Chester, United Kingdom

  2. 2

    National Cancer Institute, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 2000

Abstract

While unpurified antibodies are suitable for a number of applications, purified antibodies are required for assays based on known concentration of antibody, for chemical modifications such as radiolabeling or conjugation with fluorochromes, or for structural modifications such as production of F(ab')2 or monvalent Fab fragments. This unit contains protocols for purification of IgG by ammonium sulfate precipitation coupled with size-exclusion chromatography, Protein A- and Protein G-affinity chromatography, immunoaffinity chromatography, and ion-exchange chromatography.