UNIT 16.4 Fragmentation of Immunoglobulin G
Published Online: 1 FEB 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Andrew, S. M. and Titus, J. A. 2003. Fragmentation of Immunoglobulin G. Current Protocols in Cell Biology. 17:16.4:16.4.1–16.4.10.
- Published Online: 1 FEB 2003
- Published Print: JAN 2003
For some purposes, fragments of the IgG molecule are preferred. The Fc portion is useful for studies of biological effect–binding to the Fc receptor, mediating antibody-dependent cellular cytotoxicity, and complement fixation. The bivalent F(ab')2 produced by digestion with pepsin and the monovalent Fab produced by digestion with papain are useful for studies based on the interaction between antibody binding site(s) with antigen. This unit also describes alternative methods for preparing F(ab')2 fragments.