UNIT 16.4 Fragmentation of Immunoglobulin G

  1. Sarah M. Andrew1,
  2. Julie A. Titus2

Published Online: 1 FEB 2003

DOI: 10.1002/0471143030.cb1604s17

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Andrew, S. M. and Titus, J. A. 2003. Fragmentation of Immunoglobulin G. Current Protocols in Cell Biology. 17:16.4:16.4.1–16.4.10.

Author Information

  1. 1

    Chester College, Chester, United Kingdom

  2. 2

    National Cancer Institute, Bethesda, Maryland

Publication History

  1. Published Online: 1 FEB 2003
  2. Published Print: JAN 2003


For some purposes, fragments of the IgG molecule are preferred. The Fc portion is useful for studies of biological effect–binding to the Fc receptor, mediating antibody-dependent cellular cytotoxicity, and complement fixation. The bivalent F(ab')2 produced by digestion with pepsin and the monovalent Fab produced by digestion with papain are useful for studies based on the interaction between antibody binding site(s) with antigen. This unit also describes alternative methods for preparing F(ab')2 fragments.