Unit

UNIT 16.6 Production of Antibodies That Recognize Specific Tyrosine-Phosphorylated Peptides

  1. Michael P. DiGiovanna1,
  2. Robert R. Roussel2,
  3. David F. Stern1

Published Online: 1 FEB 2002

DOI: 10.1002/0471143030.cb1606s13

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

DiGiovanna, M. P., Roussel, R. R. and Stern, D. F. 2002. Production of Antibodies That Recognize Specific Tyrosine-Phosphorylated Peptides. Current Protocols in Cell Biology. 13:16.6:16.6.1–16.6.18.

Author Information

  1. 1

    Yale University School of Medicine, New Haven, Connecticut

  2. 2

    Dartmouth College, Hanover, New Hampshire

Publication History

  1. Published Online: 1 FEB 2002
  2. Published Print: JAN 2002

Abstract

Phosphorylation of tyrosine residues of various proteins plays an important role in regulation of the functional activities of proteins within the cell. Antibodies to specific tyrosine-phosphorylated peptides are a valuable tool for analysis of the localization and function of individual phosphoproteins. This unit describes the methods used to produce and screen monoclonal and polyclonal antibodies for specificity for only the phosphorylated state of a peptide.