UNIT 17.3 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Golemis, E. A., Serebriiskii, I., Finley, R. L., Kolonin, M. G., Gyuris, J. and Brent, R. 2001. Interaction Trap/Two-Hybrid System to Identify Interacting Proteins. Current Protocols in Cell Biology. 8:17.3:17.3.1–17.3.42.
- Published Online: 1 MAY 2001
- Published Print: OCT 2000
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This unit presents protocols designed to detect interacting proteins. Using yeast as a “test tube” and transcriptional activation of a reporter system, interacting proteins can be identified. The system can also be used to test complex formation for proteins for which there exists a reason to expect interaction.