Unit

UNIT 17.3 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins

  1. Erica A. Golemis1,
  2. Ilya Serebriiskii1,
  3. Russell L. Finley Jr.2,
  4. Mikhail G. Kolonin (hunt by interaction mating)2,
  5. Jeno Gyuris3,
  6. Roger Brent4

Published Online: 1 MAY 2001

DOI: 10.1002/0471143030.cb1703s08

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Golemis, E. A., Serebriiskii, I., Finley, R. L., Kolonin, M. G., Gyuris, J. and Brent, R. 2001. Interaction Trap/Two-Hybrid System to Identify Interacting Proteins. Current Protocols in Cell Biology. 8:17.3:17.3.1–17.3.42.

Author Information

  1. 1

    Fox Chase Cancer Center, Philadelphia, Pennsylvania

  2. 2

    Wayne State University School of Medicine, Detroit, Michigan

  3. 3

    Mitotix, Inc., Cambridge, Massachusetts

  4. 4

    The Molecular Sciences Institute, Berkeley, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 2000

This is not the most recent version of the article. View current version (1 DEC 2011)

Abstract

This unit presents protocols designed to detect interacting proteins. Using yeast as a “test tube” and transcriptional activation of a reporter system, interacting proteins can be identified. The system can also be used to test complex formation for proteins for which there exists a reason to expect interaction.