Unit

UNIT 17.6 Measuring Protein Interactions by Optical Biosensors

  1. Peter Schuck1,
  2. Lisa F. Boyd1,
  3. Peter S. Andersen2

Published Online: 1 MAY 2004

DOI: 10.1002/0471143030.cb1706s22

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Schuck, P., Boyd, L. F. and Andersen, P. S. 2004. Measuring Protein Interactions by Optical Biosensors. Current Protocols in Cell Biology. 22:17.6:17.6.1–17.6.22.

Author Information

  1. 1

    National Institutes of Health, Bethesda, Maryland

  2. 2

    University of Maryland, Rockville, Maryland

Publication History

  1. Published Online: 1 MAY 2004
  2. Published Print: MAR 2004

Abstract

In recent years, optical evanescent wave biosensors have been used to characterize protein-protein interactions, including determination of equilibrium binding constants and bimolecular rate constants. This surface binding technique can provide information about chemical on-rate constants, the lifetimes of complexes formed, and the time course of the signal. This unit provides a thorough, well-illustrated discussion of the principles of optical biosensors, experimental design, ligand immobilization, experimental data analysis, and common obstacles and possible solutions.