Unit

UNIT 17.11 Visualization of RNA Using Fluorescence Complementation Triggered by Aptamer-Protein Interactions (RFAP) in Live Bacterial Cells

  1. Maria Valencia-Burton,
  2. Natalia E. Broude

Published Online: 1 DEC 2007

DOI: 10.1002/0471143030.cb1711s37

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Valencia-Burton, M. and Broude, N. E. 2007. Visualization of RNA Using Fluorescence Complementation Triggered by Aptamer-Protein Interactions (RFAP) in Live Bacterial Cells. Current Protocols in Cell Biology. 37:17.11:17.11.1–17.11.20.

Author Information

  1. Center for Advanced Biotechnology, College of Engineering, Department of Biomedical Engineering, Boston University, Boston, Massachusetts

Publication History

  1. Published Online: 1 DEC 2007
  2. Published Print: DEC 2007

Abstract

This unit describes a method allowing RNA visualization in live cells. The method is based on fluorescent protein complementation regulated by RNA-aptamer/RNA-binding protein interactions. Based on these two principles, a fluorescent ribonucleoprotein complex is assembled inside the cell only in response to the presence of the aptamer sequence on the target RNA. Curr. Protoc. Cell Biol. 37:17.11.1-17.11.20. © 2007 by John Wiley & Sons, Inc.

Keywords:

  • protein complementation;
  • aptamer-protein interactions;
  • RNA localization;
  • fluorescent proteins;
  • eukaryotic initiation factor 4A;
  • bacterial cells