UNIT 17.11 Visualization of RNA Using Fluorescence Complementation Triggered by Aptamer-Protein Interactions (RFAP) in Live Bacterial Cells
Published Online: 1 DEC 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Valencia-Burton, M. and Broude, N. E. 2007. Visualization of RNA Using Fluorescence Complementation Triggered by Aptamer-Protein Interactions (RFAP) in Live Bacterial Cells. Current Protocols in Cell Biology. 37:17.11:17.11.1–17.11.20.
- Published Online: 1 DEC 2007
- Published Print: DEC 2007
This unit describes a method allowing RNA visualization in live cells. The method is based on fluorescent protein complementation regulated by RNA-aptamer/RNA-binding protein interactions. Based on these two principles, a fluorescent ribonucleoprotein complex is assembled inside the cell only in response to the presence of the aptamer sequence on the target RNA. Curr. Protoc. Cell Biol. 37:17.11.1-17.11.20. © 2007 by John Wiley & Sons, Inc.
- protein complementation;
- aptamer-protein interactions;
- RNA localization;
- fluorescent proteins;
- eukaryotic initiation factor 4A;
- bacterial cells