Unit

UNIT 17.14 Chromatin Immunoprecipitation of Adult Murine Cardiomyocytes

  1. P. Bolli1,
  2. C. Vardabasso2,
  3. E. Bernstein2,
  4. H.W. Chaudhry1

Published Online: 1 MAR 2013

DOI: 10.1002/0471143030.cb1714s58

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Bolli, P., Vardabasso, C., Bernstein, E. and Chaudhry, H. 2013. Chromatin Immunoprecipitation of Adult Murine Cardiomyocytes. Current Protocols in Cell Biology. 58:17.14:17.14.1–17.14.16.

Author Information

  1. 1

    Department of Cardiology, Mount Sinai School of Medicine, New York, New York

  2. 2

    Departments of Oncological Sciences and Dermatology, Mount Sinai School of Medicine, New York, New York

Publication History

  1. Published Online: 1 MAR 2013
  2. Published Print: MAR 2013

Abstract

This unit describes a streamlined two-step protocol for the isolation of adult murine cardiomyocytes with subsequent Chromatin ImmunoPrecipitation (ChIP). Isolation and culturing of cardiomyocytes is a delicate process and the protocol presented here optimizes the combination of cardiomyocyte isolation with ChIP. ChIP is an invaluable method for analyzing molecular interactions occurring between a specific protein (or its post-translationally modified form) and a region of genomic DNA. ChIP has become a widely used technique in the last decade since several groundbreaking studies have focused attention on epigenetics and have identified many epigenetic regulatory mechanisms. However, epigenetics within cardiovascular biology is a new area of focus for many investigators, and we have optimized a method for performing ChIP in adult murine cardiomyocytes, as we feel this will be an important aid to both the cardiovascular field and for the development of cell- and tissue-specific ChIP. Curr. Protoc. Cell Biol. 58:17.14.1-17.14.16. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • Chromatin ImmunoPrecipitation;
  • adult murine cardiomyocytes;
  • cardiomyocyte isolation