Unit

UNIT 18.5 Analysis of Mitochondrial Dysfunction During Cell Death

  1. Vladimir Gogvadze,
  2. Sten Orrenius,
  3. Boris Zhivotovsky

Published Online: 1 AUG 2003

DOI: 10.1002/0471143030.cb1805s19

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Gogvadze, V., Orrenius, S. and Zhivotovsky, B. 2003. Analysis of Mitochondrial Dysfunction During Cell Death. Current Protocols in Cell Biology. 19:18.5:18.5.1–18.5.27.

Author Information

  1. Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden

Publication History

  1. Published Online: 1 AUG 2003
  2. Published Print: JUN 2003

Abstract

Attempts to identify a common underlying step in the apoptotic program in response to various cytotoxic stimuli have focused on the role of mitochondria in this form of cell death. This unit contains a family of protocols that can be used to assess mitochondrial functions during apoptotic responses. Protocols are included for the collection and analysis of released proteins, for detection of the mitochondrial permeability transition, for measurement of mitochondrial membrane potential, and for preparation of mitochondria from different tissue sources.