Unit

UNIT 19.7 Imaging Tumor Cell Movement In Vivo

  1. Dmitriy Kedrin1,
  2. Jeffrey Wyckoff1,
  3. Erik Sahai2,
  4. John Condeelis1,
  5. Jeffrey E. Segall1

Published Online: 1 JUN 2007

DOI: 10.1002/0471143030.cb1907s35

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Kedrin, D., Wyckoff, J., Sahai, E., Condeelis, J. and Segall, J. E. 2007. Imaging Tumor Cell Movement In Vivo. Current Protocols in Cell Biology. 35:19.7:19.7.1–19.7.17.

Author Information

  1. 1

    Albert Einstein College of Medicine, Bronx, New York

  2. 2

    London Research Institute, London, England

Publication History

  1. Published Online: 1 JUN 2007
  2. Published Print: JUN 2007

This is not the most recent version of the article. View current version (1 MAR 2013)

Abstract

This unit describes the methods that we have been developing for analyzing tumor cell motility in mouse and rat models of breast cancer metastasis. Rodents are commonly used to provide a mammalian system for studying human tumor cells as xenografts in immunocompromised mice, as well as for following the development of tumors from a specific tissue type in transgenic lines. The Basic Protocol describes the standard methods used for generation of mammary tumors and imaging them. Additional protocols for labeling macrophages, blood vessel imaging, and image analysis are also included.

Keywords:

  • Imaging;
  • metastasis;
  • mammary tumor;
  • image analysis