Unit

UNIT 19.7 Imaging Tumor Cell Movement In Vivo

  1. David Entenberg1,3,
  2. Dmitriy Kedrin1,3,
  3. Jeffrey Wyckoff1,
  4. Erik Sahai2,
  5. John Condeelis1,
  6. Jeffrey E. Segall1

Published Online: 1 MAR 2013

DOI: 10.1002/0471143030.cb1907s58

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Entenberg, D., Kedrin, D., Wyckoff, J., Sahai, E., Condeelis, J. and Segall, J. E. 2013. Imaging Tumor Cell Movement In Vivo. Current Protocols in Cell Biology. 58:19.7:19.7.1–19.7.19.

Author Information

  1. 1

    Albert Einstein College of Medicine, Bronx, New York

  2. 2

    London Research Institute, London, United Kingdom

  3. 3

    These authors contributed equally to this work.

Publication History

  1. Published Online: 1 MAR 2013
  2. Published Print: MAR 2013

Abstract

This unit describes the methods that we have been developing for analyzing tumor cell motility in mouse and rat models of breast cancer metastasis. Rodents are commonly used both to provide a mammalian system for studying human tumor cells (as xenografts in immunocompromised mice) as well as for following the development of tumors from a specific tissue type in transgenic lines. The Basic Protocol in this unit describes the standard methods used for generation of mammary tumors and imaging them. Additional protocols for labeling macrophages, blood vessel imaging, and image analysis are also included. Curr. Protoc. Cell Biol. 58:19.7.1-19.7.19. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • imaging;
  • metastasis;
  • mammary tumor;
  • image analysis