UNIT 19.10 Overview: Engineering Transgenic Constructs and Mice
Published Online: 1 MAR 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Haruyama, N., Cho, A. and Kulkarni, A. B. 2009. Overview: Engineering Transgenic Constructs and Mice. Current Protocols in Cell Biology. 42:19.10:19.10.1–19.10.9.
- Published Online: 1 MAR 2009
- Published Print: MAR 2009
Cell biology research encompasses everything from single cells to whole animals. Recent discoveries concerning particular gene functions can be applied to the whole animal for understanding genotype-phenotype relationships underlying disease mechanisms. For this reason, genetically manipulated mouse models are now considered essential to correctly understand disease processes in whole animals. This unit reviews the basic mouse technologies used to generate conventional transgenic mice, which represent a gain-of-function approach. First, an overview of transgenic construct design is presented. This unit then explains basic strategies for the identification and establishment of independent transgenic mouse lines, followed by comments on historical and emerging techniques. It then describes typical problems that are encountered when researchers start to generate transgenic mice. Curr. Protoc. Cell Biol. 42:19.10.1-19.10.9. © 2009 by John Wiley & Sons, Inc.
- transgenic mice;
- plasmid vector;
- gene expression;
- reporter gene