UNIT 19.11 Generation of Transgenic Mice

  1. Andrew Cho,
  2. Naoto Haruyama,
  3. Ashok B. Kulkarni

Published Online: 1 MAR 2009

DOI: 10.1002/0471143030.cb1911s42

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Cho, A., Haruyama, N. and Kulkarni, A. B. 2009. Generation of Transgenic Mice. Current Protocols in Cell Biology. 42:19.11:19.11.1–19.11.22.

Author Information

  1. National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAR 2009
  2. Published Print: MAR 2009


This unit describes detailed step-by-step protocols, reagents, and equipment required for successful generation of transgenic mice using pronuclear injection. The experimental methods and practical tips given here will help guide beginners in understanding what is required and what to avoid in these standard protocols for efficiently generating transgenic mice. Curr. Protoc. Cell Biol. 42:19.11.1-19.11.22. © 2009 by John Wiley & Sons, Inc.


  • DNA purification;
  • genotyping;
  • embryo harvesting;
  • embryo implantation;
  • transgenic mice;
  • zygote