UNIT 19.11 Generation of Transgenic Mice
Published Online: 1 MAR 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Cho, A., Haruyama, N. and Kulkarni, A. B. 2009. Generation of Transgenic Mice. Current Protocols in Cell Biology. 42:19.11:19.11.1–19.11.22.
- Published Online: 1 MAR 2009
- Published Print: MAR 2009
This unit describes detailed step-by-step protocols, reagents, and equipment required for successful generation of transgenic mice using pronuclear injection. The experimental methods and practical tips given here will help guide beginners in understanding what is required and what to avoid in these standard protocols for efficiently generating transgenic mice. Curr. Protoc. Cell Biol. 42:19.11.1-19.11.22. © 2009 by John Wiley & Sons, Inc.
- DNA purification;
- embryo harvesting;
- embryo implantation;
- transgenic mice;