UNIT 19.13 Manipulation of Mouse Embryonic Stem Cells for Knockout Mouse Production

  1. Advait Limaye,
  2. Bradford Hall,
  3. Ashok B. Kulkarni

Published Online: 1 SEP 2009

DOI: 10.1002/0471143030.cb1913s44

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Limaye, A., Hall, B. and Kulkarni, A. B. 2009. Manipulation of Mouse Embryonic Stem Cells for Knockout Mouse Production. Current Protocols in Cell Biology. 44:19.13:19.13.1–19.13.24.

Author Information

  1. National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland

Publication History

  1. Published Online: 1 SEP 2009
  2. Published Print: SEP 2009


The establishment of mouse embryonic stem (ES) cell lines has allowed for the gene?ration of the knockout mouse. ES cells that are genetically altered in culture can then be manipulated to derive a whole mouse containing the desired mutation. To successfully generate a knockout mouse, however, the ES cells must be carefully cultivated in a pluripotent state throughout the gene-targeting experiment. This unit describes detailed step-by-step protocols, reagents, equipment, and strategies needed for the successful generation of gene knockout embryonic stem cells using homologous recombination technologies. Curr. Protoc. Cell Biol. 44:19.13.1-19.13.24. © 2009 by John Wiley & Sons, Inc.


  • mouse embryonic stem cells;
  • homologous recombination