Unit

UNIT 19.14 Generation of Gene Knockout Mice by ES Cell Microinjection

  1. Glenn Longenecker,
  2. Ashok B. Kulkarni

Published Online: 1 SEP 2009

DOI: 10.1002/0471143030.cb1914s44

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Longenecker, G. and Kulkarni, A. B. 2009. Generation of Gene Knockout Mice by ES Cell Microinjection. Current Protocols in Cell Biology. 44:19.14:19.14.1–19.14.36.

Author Information

  1. National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland

Publication History

  1. Published Online: 1 SEP 2009
  2. Published Print: SEP 2009

Abstract

This unit describes protocols used in the production of chimeric mice that are then used for the generation of gene knockout mice. These protocols include the collection of blastocyst embryos, ES cell injection, and uterine transfer of injected blastocysts. Support protocols for the superovulation of blastocyst donor mice, generation of pseudopregnant recipients, fabrication of glass pipets for embryo and cell manipulations, and generation of germline mice are also included. Practical tips and solutions are mentioned to help troubleshoot problems that may occur. Curr. Protoc. Cell Biol. 44:19.14.1.-19.14.36. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • embryonic stem cells;
  • blastocyst;
  • blastocyst injection;
  • uterine transfer;
  • chimeric mice;
  • knockout mice