UNIT 19.14 Generation of Gene Knockout Mice by ES Cell Microinjection
Published Online: 1 SEP 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Longenecker, G. and Kulkarni, A. B. 2009. Generation of Gene Knockout Mice by ES Cell Microinjection. Current Protocols in Cell Biology. 44:19.14:19.14.1–19.14.36.
- Published Online: 1 SEP 2009
- Published Print: SEP 2009
This unit describes protocols used in the production of chimeric mice that are then used for the generation of gene knockout mice. These protocols include the collection of blastocyst embryos, ES cell injection, and uterine transfer of injected blastocysts. Support protocols for the superovulation of blastocyst donor mice, generation of pseudopregnant recipients, fabrication of glass pipets for embryo and cell manipulations, and generation of germline mice are also included. Practical tips and solutions are mentioned to help troubleshoot problems that may occur. Curr. Protoc. Cell Biol. 44:19.14.1.-19.14.36. © 2009 by John Wiley & Sons, Inc.
- embryonic stem cells;
- blastocyst injection;
- uterine transfer;
- chimeric mice;
- knockout mice