Unit

UNIT 20.5 Transfection by Electroporation

  1. Huntington Potter1,
  2. Richard Heller2,3

Published Online: 1 SEP 2011

DOI: 10.1002/0471143030.cb2005s52

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Potter, H. and Heller, R. 2011. Transfection by Electroporation. Current Protocols in Cell Biology. 52:20.5:20.5.1–20.5.10.

Author Information

  1. 1

    Byrd Alzheimer's Institute, University of South Florida College of Medicine, Tampa, Florida

  2. 2

    Medical Laboratory and Radiation Sciences, Old Dominion University, Norfolk, Virginia

  3. 3

    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, Virginia

Publication History

  1. Published Online: 1 SEP 2011
  2. Published Print: SEP 2011

Abstract

Electroporation—the use of high-voltage electric shocks to introduce DNA into cells—can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques. This unit describes electroporation of mammalian cells, including ES cells for the preparation of knock-out, knock-in, and transgenic mice. Protocols are described for the use of electroporation in vivo to perform gene therapy for cancer therapy and DNA vaccination. Also described are modifications for preparation and transfection of plant protoplasts. Curr. Protoc. Cell Biol. 52:20.5.1-20.5.10. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • molecular biology;
  • introduction of DNA into cells;
  • gene regulation;
  • gene expression;
  • transcription and translation;
  • gene therapy;
  • DNA vaccine