Unit

UNIT 20.6 Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents

  1. Pamela Hawley-Nelson,
  2. Valentina Ciccarone

Published Online: 1 AUG 2003

DOI: 10.1002/0471143030.cb2006s19

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Hawley-Nelson, P. and Ciccarone, V. 2003. Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents. Current Protocols in Cell Biology. 20:20.6:20.6.1–20.6.17.

Author Information

  1. Life Technologies, Inc., Rockville, Maryland

Publication History

  1. Published Online: 1 AUG 2003
  2. Published Print: JUN 2003

Abstract

The development of high-efficiency methods for the introduction of functional genetic material into eukaryotic cells using cationic lipids has accelerated biological research in the studies of gene expression, control of cell growth, and cell lineage. Transfection mediated by cationic lipids is commonly used in industrial protein production as well as in some clinical gene therapy protocols. Replacing our previous unit on this topic, this new version describes how to perform transfection of adherent and suspension cells, insect cells, and RNA transfection using the cationic lipid system.