UNIT 20.7 Optimization of Transfection

  1. John K. Rose

Published Online: 1 AUG 2003

DOI: 10.1002/0471143030.cb2007s19

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Rose, J. K. 2003. Optimization of Transfection. Current Protocols in Cell Biology. 19:20.7:20.7.1–20.7.4.

Author Information

  1. Yale University School of Medicine, New Haven, Connecticut

Publication History

  1. Published Online: 1 AUG 2003
  2. Published Print: JUN 2003


When embarking upon any transfection procedure, a critical first step is to optimize conditions. Every mammalian cell type has a characteristic set of requirements for optimal introduction of foreign DNA; there is a tremendous degree of variability in the transfection conditions that work, even among cell types that are very similar to one another. Often, an experimenter must screen a wide variety of cell types for a desired regulatory trait, such as an appropriate response to a particular effector molecule. It is thus helpful to have a straightforward, systematic approach to optimizing transfection efficiency. This unit provides guidelines to optimizing transfection experiments.