UNIT 20.7 Optimization of Transfection
Published Online: 1 AUG 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
How to Cite
Rose, J. K. 2003. Optimization of Transfection. Current Protocols in Cell Biology. 19:20.7:20.7.1–20.7.4.
- Published Online: 1 AUG 2003
- Published Print: JUN 2003
When embarking upon any transfection procedure, a critical first step is to optimize conditions. Every mammalian cell type has a characteristic set of requirements for optimal introduction of foreign DNA; there is a tremendous degree of variability in the transfection conditions that work, even among cell types that are very similar to one another. Often, an experimenter must screen a wide variety of cell types for a desired regulatory trait, such as an appropriate response to a particular effector molecule. It is thus helpful to have a straightforward, systematic approach to optimizing transfection efficiency. This unit provides guidelines to optimizing transfection experiments.