Unit

UNIT 21.6 Photoactivation and Imaging of Photoactivatable Fluorescent Proteins

  1. George H. Patterson

Published Online: 1 MAR 2008

DOI: 10.1002/0471143030.cb2106s38

Current Protocols in Cell Biology

Current Protocols in Cell Biology

How to Cite

Patterson, G. H. 2008. Photoactivation and Imaging of Photoactivatable Fluorescent Proteins. Current Protocols in Cell Biology. 38:21.6:21.6.1–21.6.10.

Author Information

  1. Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAR 2008
  2. Published Print: MAR 2008

Abstract

A major advance in the microscopic study of cells and tissues is the introduction of photoactivatable fluorescent proteins, which can specifically mark proteins of interest within a living cell. Fluorescent proteins are now available that allow a pool of molecules to be “turned on” by photoactivation. This unit discusses technical aspects for the general use of photoactivatable fluorescent proteins and introduces some specific applications in the concluding remarks. Curr. Protoc. Cell Biol. 38:21.6.1-21.6.10. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • photoactivatable;
  • fluorescent protein;
  • microscopy